OPTIMIZATION OF ISOFLAVONES PRODUCTION  ON CALLUS (Pachyrhyzus et al.) THROUGH VARIATIONS IN SOURCES AND AMOUNT OF PLANTING MATERIAL

Indarwati Indarwati; Jajuk  Herawati; Dwie Retna Soeryaningsih; Surya Ari  Widya; Faez M. Hassan

doi:10.53067/ijomral.v3i6.281

Authors

Indarwati Indarwati Wijaya Kusuma University
Jajuk Herawati Wijaya Kusuma University
Dwie Retna Soeryaningsih Wijaya Kusuma University
Surya Ari Widya Wijaya Kusuma University
Faez M. Hassan Mustansiriyah University

DOI:

https://doi.org/10.53067/ijomral.v3i6.281

Keywords:

Invitro, Secondary Metabolites, Isoflavon, Pachyrhizus erosus

Abstract

In vitro, biotechnology has proven to be a reliable source for producing secondary metabolites. Isoflavones are secondary plant metabolites that have antioxidant and estrogenic properties in the body. Tissue culture technology, which allows the production of plantlets or calluses in small containers, can address challenges in plant metabolite production. To enhance the efficiency of callus production, variations in sources and amount of planting material should be tested in a single planting container. The Bengkoang plant / Jicama (Pachyrhizus erosus) naturally produces isoflavones. This study investigates the impact of variations in sources and amount of planting material on the production of isoflavones in Bengkoang callus using in vitro tissue culture techniques. The research was conducted at the Tissue Culture Laboratory, employing a Completely Randomized Design (CRD) factorial method. Factor I involved the explant source: C1: young leaves; C2: Callus. Factor II involved the number of explants per container: T1: 1; T2: 2; T3: 3. The results show an interaction between source variations and the amount of planting material in anthocyanin production, successfully extracted from the Callus. Planting 3 explants in a container with subculture material from Callus produced the highest 0.16% isoflavones compared to other treatments

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References

Abdulhafiz, F., Reduan, M. F. H., Hamzah, Z., Kari, Z. A., Dawood, M. A., & Mohammed, A. (2022). Acute oral toxicity assessment and anti-hyperuricemic activity of Alocasia longiloba extract on Sprague-Dawley rats. Saudi Journal of Biological Sciences, 29(5), 3184-3193.

Aidah, S. N., & Indonesia, T. P. K. 2020. Ensiklopedi Bengkuang: Deskripsi, Filosofi, Manfaat, Budidaya dan Peluang Bisnisnya (Vol. 7). Penerbit KBM Indonesia.

Chandran H, Meena M, Barupal T, Sharma K. Plant tissue culture as a perpetual source for producing industrially critical bioactive compounds. Biotechnol Rep. 2020;26:00450.

Cseke, L.J., Kirakosyan, A., Kaufman, P.B., Warber, S., Duke, J.A. and Brielmann, H.L., 2016. Natural products from plants. CRC press.

Dyshlyuk, A. D. Vesnina, A. I. Dmitrieva, O. V Kozlova, and A. Y. Prosekov, “Ptimización De Parámetros Para La Obtención De Cultivos De Callos, Suspensiones Y Raíces De Reina De Los Prados (Filipendula Ulmaria) Para Aislar El Mayor Número De Sustancias Biológicamente Activas Con Propiedades Geroprotectora,” vol. 84, pp. 1–14, 2021.

Fazili, M.A., Bashir, I., Ahmad, M., Yaqoob, U. and Geelani, S.N., (2022). In vitro strategies for enhancing secondary metabolite production in plants: a review. Bulletin of the National Research Centre, 46(1), 35.

Habibah, N. A., Safitri, S., Pratiwi, Y. R., Wijawati, N., Musafa, F., Puspitasari, A. S. (2021). Callus induction from the tuber of lesser yam (Dioscorea esculenta) on MS media supplemented by 2,4-D and kinetin. Journal of Physics: Conference Series 1918 052029. https://doi.org/10.1088/1742-6596/1918/5/052029

Hariyati, M., Bachtiar, I., & Sedijani, P. (2016). Induksi kalus tanaman krisan (Chrysanthemum morifolium) dengan pemberian benzil amino purin (BAP) dan diklorofenoksi acetil acid (2,4- D). Jurnal Penelitian Pendidikan IPA 2(1), 89-96. https://doi.org/10.29303/jppipa.v2i1.37

https://link.springer.com/article/10.1186/s42269-022-00717-z

Indah, P. N., dan Ermavitalini, D., 2013. Induksi Kalus Daun Nyamplung (Calophyllum et al.) pada Beberapa Kombinasi Konsentrasi6-Benzylaminopurine (BAP) dan 2, 4-Dichlorophenoxyacetic Acid (2, 4-D). Jurnal Sains dan Seni ITS. 2.E1-E6

Indarwati, I.“In Vitro Culture of Dragon Fruit (Hylocereus polyrhizus): Callus and Anthocyanin Production,” Agrotech J., vol. 6, no. 2, pp. 78–86, 2022, doi: 10.31327/atj.v6i2.1659

Lukitaningsih, E. and U. Holzgrabe. 2014. “Bioactive Compounds In Bengkoang (Pachyrhizus Erosus) As Antioxidant And Tyrosinase Inhibiting Agents,” Indones. J. Pharm., vol. 25, no. 2, p. 68, 2014, doi: 10.14499/indonesianjpharm25iss2pp68-75

Lutfiah, A., & Habibah, N. A. (2022). Pengaruh Pemberian Elisitor Ekstrak Khamir pada Pertumbuhan Kultur Kalus Gembili dengan Penambahan ZPT 2, 4-D dan Kinetin. Indonesian Journal of Mathematics and Natural Sciences, 45(2), 77-83.

Ningsih, I. Y. (2014). Pengaruh elisitor biotik dan abiotik pada produksi flavonoid melalui kultur jaringan tanaman. Pharmacy 11(2), https://doi.org/117-132. 10.30595/pji.v11i2.829

Pan Y, Li L, Xiao S, Chen Z, Sarsaiya S, Zhang S, Guan YS, Liu H, Xu D (2020) Callus growth kinetics and accumulation of Bletilla striata Rchb secondary metabolites. f. using a callus suspension culture. PLoS ONE 10:1–14

Priyanka, V., Kumar, R., Dhaliwal, I. and Kaushik, P., 2021. Germplasm conservation: instrumental in agricultural biodiversity a review. Sustainability, 13(12), p.6743.

Shafi, A., Hassan, F., Zahoor, I., Majeed, U. & Khanday, F.A. (2021). Biodiversity, management, and sustainable use of medicinal and aromatic plant resources. Medicinal and aromatic plants: healthcare and industrial applications, pp.85–111.

Sharma S, Sharma S, Sharma V (2021). Exploring In-vitro micropropagation and secondary metabolite production in Desmodium gangeticum (L.) D.C. I.K. Press 22:231–240

Sujono, T.A., Nurrochmad, A., Lukitaningsih, E. and Nugroho, A.E., 2021. Immunomodulatory effect of methanolic extract and ethyl acetate fraction of Sengkang (Pachyrhizus erosus (L.) Urban) tuber in mice. Pharmacognosy Journal, 13(1)

Tan, A.E., Kusuma, C.P., Gunawan, C.C., Natasha, E., Fiona, F., Lidharta, J.G., Wijaya, R.O., Thang, R.C. and Karema, S.M., 2021. Kultur Jaringan Tanaman dan Aplikasinya Dalam Dunia Bisnis.

Twaij, B. M., Jazar, Z. H., & Hasan, M. N. (2020). Trends in the use of tissue culture, applications, and future aspects. International Journal of Plant Biology, 11(1), 8385